Tsigenopoulos Constantinos
Position: Researcher, Grade B
Contact Info
Institute: Marine Biology, Biotechnology and Aquacu
Address: Thalassocosmos, Ex-US Base at Gournes Pediados, 715 00 Heraklion, Crete
Email:tsigeno@hcmr.gr
Tel: +30 2810 337854
Telex: +30 2810 337820
Website: http://www.hcmr.gr
Dr. Costas Tsigenopoulos received a B.Sc degree in Biology from the Aristotle University of Thessaloniki, an MSc and a Ph.D in Evolutionary Biology and Ecology, from the University of Montpellier II in France (1995-1999).
Dr. Tsigenopoulos research interests and experience include mainly linkage mapping, comparative genomics, phylogenetics and phylogeography, use of microsatellite and SNP genetic markers, mtDNA sequencing, ploidy evolution in vertebrates,and in the past karyological studies, allozymes and protein electrophoresis.
He is currently interested in the use of molecular genetic markers to i) address questions of population structure of natural marine stocks, and ii) assist genetic improvement in aquaculture. The former work has yielded some very successful applications of molecular markers in studies of the genetic structure of marine species while the latter has lead to the development and application of molecular genetic markers (microsatellite DNA) for parentage identification in Mediterranean cultivated fish species (Dicentrarchus labrax and Sparus aurata). Recently, he is interested also in comparative genomics and the introduction of genomic approaches in aquaculture practices and is actively contributing to practical applications of the produced results in commercial farms.
Dr. Tsigenopoulos research interests and experience include mainly linkage mapping, comparative genomics, phylogenetics and phylogeography, use of microsatellite and SNP genetic markers, mtDNA sequencing, ploidy evolution in vertebrates,and in the past karyological studies, allozymes and protein electrophoresis.
He is currently interested in the use of molecular genetic markers to i) address questions of population structure of natural marine stocks, and ii) assist genetic improvement in aquaculture. The former work has yielded some very successful applications of molecular markers in studies of the genetic structure of marine species while the latter has lead to the development and application of molecular genetic markers (microsatellite DNA) for parentage identification in Mediterranean cultivated fish species (Dicentrarchus labrax and Sparus aurata). Recently, he is interested also in comparative genomics and the introduction of genomic approaches in aquaculture practices and is actively contributing to practical applications of the produced results in commercial farms.
Education Profile
1999, PhD (Thèse) ) at the Université Montpellier II Sciences et Techniques du Languedoc, Montpellier, France. École Doctorale: Biologie des Populations et Ecologie.
Research project entitled: “ Phylogéographie du genre Barbus dans les régions péri- méditerranéennes. Reconstitutions des grandes lignées de migration en moyen des marqueurs nucléaires et mitochondriaux” / “Phylogeography of species of the genus Barbus (Teleosts, Cyprinidae) in the Mediterranean region. Reconstitution of the major migration lineages by means of nuclear and mitochondrial genetic markers”. Supervisor: Dr P. Berrebi (Laboratoire Génome et Populations, CNRS, Montpellier), p. 192
1996, MSc (DEA, Diplôme d’Etudes Approfondies) ) at the Université Montpellier II Sciences et Techniques du Languedoc– Ecole Nationale Supérieure Agronomique de Montpellier (ENSAM), Montpellier, France. Spécialisation: Biologie Evolutive et Ecologie.
Research project entitled: “Phylogéographie mitochondriale du genre Barbus au nord de la Méditerranée. Une approche de la spéciation des poissons d’eau douce d’Europe occidentale / Mitochondrial phylogeography of the genus Barbus in Northern Mediterranean. A speciation approach of freshwater fish in Western Europe ”. Supervisor: Dr P. Berrebi (Laboratoire Génome et Populations, CNRS, Montpellier), p. 58
1994, BSc (Degree in Biology) at the Aristotle University of Thessaloniki (Greece).
Research project entitled: “Phylogenetic relationships among Greek species of the genus Leuciscus (Cyprinidae, Pisces) under the direction of Dr Y. Karakousis (Laboratory of Genetics, School of Biology, AUTh), Thessaloniki, p. 46
1999, PhD (Thèse) ) at the Université Montpellier II Sciences et Techniques du Languedoc, Montpellier, France. École Doctorale: Biologie des Populations et Ecologie.
Research project entitled: “ Phylogéographie du genre Barbus dans les régions péri- méditerranéennes. Reconstitutions des grandes lignées de migration en moyen des marqueurs nucléaires et mitochondriaux” / “Phylogeography of species of the genus Barbus (Teleosts, Cyprinidae) in the Mediterranean region. Reconstitution of the major migration lineages by means of nuclear and mitochondrial genetic markers”. Supervisor: Dr P. Berrebi (Laboratoire Génome et Populations, CNRS, Montpellier), p. 192
1996, MSc (DEA, Diplôme d’Etudes Approfondies) ) at the Université Montpellier II Sciences et Techniques du Languedoc– Ecole Nationale Supérieure Agronomique de Montpellier (ENSAM), Montpellier, France. Spécialisation: Biologie Evolutive et Ecologie.
Research project entitled: “Phylogéographie mitochondriale du genre Barbus au nord de la Méditerranée. Une approche de la spéciation des poissons d’eau douce d’Europe occidentale / Mitochondrial phylogeography of the genus Barbus in Northern Mediterranean. A speciation approach of freshwater fish in Western Europe ”. Supervisor: Dr P. Berrebi (Laboratoire Génome et Populations, CNRS, Montpellier), p. 58
1994, BSc (Degree in Biology) at the Aristotle University of Thessaloniki (Greece).
Research project entitled: “Phylogenetic relationships among Greek species of the genus Leuciscus (Cyprinidae, Pisces) under the direction of Dr Y. Karakousis (Laboratory of Genetics, School of Biology, AUTh), Thessaloniki, p. 46
Professional Scientific Experience
2/2012- today: Researcher Grade B’, Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR) Crete, Heraklion.
9/2006 –1/2012: Researcher Grade C’, Institute of Marine Biology and Genetics (IMBG), Hellenic Centre for Marine Research (HCMR) Crete, Heraklion.
2/2002-9/2006: Department of Genetics and Molecular Biotechnology, Institute of Marine Biology of Crete (IMBC), Heraklion Crete, Participation in Research projects.
10/1999-7/2000: Post-Doctoral fellow, Department of Genetics and Molecular Biotechnology, Institute of Marine Biology of Crete (IMBC), Heraklion Crete, Participation in Research projects.
1. Quéré Nolwenn, Desmarais Erick, Tsigenopoulos Costas S., Belkhir Khalid, Bonhomme François & Bruno Guinand. Gene flow at major transitional areas in sea bass (Dicentrarchus labrax) and the emergence of a hybrid swarm (in press in Ecology and Evolution, ECE-2012-07-0172)
2. Chaoui L., P-A.Gagnaire, B. Guinand, J-P. Quignard, C. Tsigenopoulos, M. H. Kara & F. Bonhomme 2012. Microsatellite length variation in candidate genes correlates with habitat in the gilthead sea bream Sparus aurata. Molecular Ecology, 21: 5497–5511
3. Loukovitis D., E. Sarropoulou, C. Batargias, A. P. Apostolidis, G. Kotoulas, C. S. Tsigenopoulos & D. Chatziplis 2012. Quantitative Trait Loci for Body Growth and Sex Determination in the Hermaphrodite Teleost Fish Sparus aurata L., Animal Genetics, 43: 753-759
4. Makridis P., Ferreira T., Kokou F., Tsigenopoulos C. & P. Divanach. 2012. Quantitative and Qualitative Aspects of Bacterial Communities Associated with Cultures of Chlorella minutissima. Journal of the World Aquaculture Society, 43: 571-578
5. Limborg M T, Hanel R, Debes P V, Ring AK, André C, Tsigenopoulos CS & D Bekkevold. 2012. Disentangling evolutionary processes shaping population structure across marine transition zones: Insights from a Pan European small pelagic fish. Heredity, 109: 96-107
6. Vogiatzi E., Hanel R., Dailianis T., Lagnel J., Hassan M., Magoulas A. & C.S Tsigenopoulos. 2012. Description of microsatellite markers in four mullids based on the development and cross-species amplification of 18 new markers in red mullet (Mullus barbatus). Biochemical Systematics and Ecology, 44: 279-285
7. Coscia I, Vogiatzi E, Kotoulas G, Tsigenopoulos C S. & S Mariani. 2012. Neutral and adaptive genetic variation in expanding populations of gilthead sea bream, Sparus aurata L., in the North-East Atlantic. Heredity, 108:537-546
8. Lefkaditou E., Tsigenopoulos C. S., Alidromiti C. & Haralabous J. 2012. On the occurrence of Alloteuthis subulata in the Eastern Ionian Sea and its distinction from the sympatric Alloteuthis media. Journal of Biological Research, 17: 169-175
9. Loukovitis D., Sarropoulou E., Vogiatzi E., Tsigenopoulos C.S., Kotoulas G., Magoulas A. & Chatziplis D. 2012 Genetic variation in farmed populations of the gilthead sea bream Sparus aurata in Greece using microsatellite DNA markers. Aquaculture Research, 43: 239-246
10. Rabaoui L., Mejri R., Bahri L., Tlig-Zouari S., Ben Hassine Oum Kalthoum & C. S. Tsigenopoulos. 2011 Genetic differentiation among populations of the endangered fan mussel Pinna nobilis (Mollusca) along the Tunisian coastline. Hydrobiologia, 678: 99-111
11. Dailianis T, Tsigenopoulos C, Dounas C & Voultsiadou E. 2011. Genetic diversity of the imperilled bath sponge Spongia officinalis Linnaeus, 1759 across the Mediterranean Sea: patterns of population differentiation and implications for taxonomy and conservation. Molecular Ecology, 20(18): 3757-3772
12. Boulton K, Massault C, Houston R, de Koning DJ, Haley C, Bovenhuis H, Batargias C, Canario AVM, Kotoulas G, C S Tsigenopoulos. 2011. QTL affecting morphometric traits and stress response in the gilthead seabream (Sparus aurata). Aquaculture, 319(1-2): 58-66
13. Vogiatzi E., Lagnel J., Pakaki V., Louro B., Canario A., Reinhardt R., Kotoulas G., Magoulas A. & C. S. Tsigenopoulos. 2011. In silico mining and characterization of simple sequence repeats from gilthead sea bream (Sparus aurata) expressed sequence tags (EST-SSRs); PCR amplification, polymorphism evaluation and multiplexing and cross-species assays. Marine Genomics, 4(2): 83-91
14. Hanel R. & Tsigenopoulos C.S. 2011. Phylogeny, Evolution and Taxonomy of sparids with some notes on their Ecology and Biology: Chapter 2. In “Sea bream: Biology & Aquaculture of Sparidae”, pp. 51-74, M. Pavlidis and C.C.Mylonas Eds, Wiley-Blackwell, ISBN 978-1-4051-9772-4
15. Loukovitis D., E. Sarropoulou, C. S. Tsigenopoulos, C. Batargias A. Magoulas, A. P. Apostolidis, D. Chatziplis & G. Kotoulas. 2011. Quantitative Trait Loci involved in sex determination and body growth in the gilthead sea bream (Sparus aurata L.) through targeted genome scan. PLoS ONE 6(1): e16599. doi:10.1371/journal.pone.0016599
16. Carvalho, G.R., Creer, S., Allen, M.J., Costa, F.O., Tsigenopoulos, C.S., Le Goff-Vitry, M., Magoulas, A., Medlin, L. & Metfies, K. 2010. Genomics in the Discovery and Monitoring of Marine Biodiversity, Chapter 1. In ‘Introduction to Marine Genomics’, pp. 1-32, Cock, J.M., Tessmar-Raible, K., Boyen, C. & Viard, F. Eds, Springer, ISBN 978-90-481-8616-7
17. Louro, B.E.P.., Passos, A.L.S., Souche E., Tsigenopoulos, C. , Beck, A., L., Bonhomme, F., Cancela, L., , Cerdà, J.6, Clark, M.S., Kotoulas, G., Lubzens, E., Planas, J., Volckaert, F.A.M., Reinhardt, R. & A.V.M Canario. 2010. Gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax) expressed sequence tags from multiple tissues: characterization, tissue specific expression and gene markers. Marine Genomics, 3(3-4): 179-191
18. Katharios P. & C.S. Tsigenopoulos. 2010. Nodavirus infection in cultured juvenile shi-drum, Umbrina cirrosa, in Greece. Aquaculture Research, 42: 147-152
19. Dailianis T. & Tsigenopoulos C. S. 2010. Characterization of polymorphic microsatellite markers for the endangered Mediterranean bath sponge Spongia offcinalis L. Conservation Genetics, 11: 1155-1158
20. Tsigenopoulos C. S., Kasapidis P. & Berrebi P. 2010. Phylogenetic relationships of hexaploid large-sized barbs (genus Labeobarbus, Cyprinidae) based on mtDNA data. Molecular Phylogenetics and Evolution 56(2): 851-856
21. Vogiatzi E., Kalogianni E., Giakoumi S., Magoulas A. & Tsigenopoulos C. S. 2009. Characterization of polymorphic microsatellite markers in Valencia letourneuxi (Valenciidae) and cross-amplification in two other cyprinodontiform species. Conservation Genetics Resources 1: 27-30
22. Lagnel J., Tsigenopoulos C. S. & Iliopoulos G. 2009. Noblast and Jamblast: new options and a java application manager for BLAST Bioinformatics 25(6):824-826
23. Volckaert F.A.M., Batargias C., Canario A., Chatziplis D., Chistiakov D., Haley C., Libertini A. & Tsigenopoulos C. S. 2008. European sea bass. In “Genome Mapping and Genomics in Fishes and Aquatic animals”, pp. 117-134. Thomas D. Kocher and Chittaranjan Kole Eds., Springer Verlag, ISBN 978-3-540-73836-7
24. Kyriazi P., N. Poulakakis, A. Parmakelis, P.A Crochet, J. Moravec, N. Rastegar-Pouyani, C. S. Tsigenopoulos, A. Magoulas, M. Mylonas & Lymberakis P. 2008. Mitochondrial DNA reveals the genealogical history of the Snake-eyed lizards (Ophisops elegans and O. occidentalis) (Sauria: Lacertidae). Molecular Phylogenetics and Evolution 49: 795–805
25. Chistiakov D.A., Tsigenopoulos C.S., Lagnel J., Guo Y.M., Hellemans B., Haley C.S., Volckaert F.A.M. & Kotoulas G. 2008. A combined AFLP and microsatellite linkage map and pilot comparative genomic analysis of European sea bass Dicentrarchus labrax L. Animal Genetics 39: 623-634
26. Dailianis, T., Limborg, M., Hanel, R., Bekkevold, D., Lagnel, J., Magoulas, A. and Tsigenopoulos, C. S. 2008 Characterization of nine polymorphic microsatellite markers in sprat (Sprattus sprattus L.). Molecular Ecology Resources 8: 861-863.
27. Apostolidis A., Loukovitis D. & Tsigenopoulos C. S. 2008. Genetic characterization of brown trout (Salmo trutta) populations from the Southern Balkans using mtDNA sequencing and RFLP analysis. Hydrobiologia 600: 169-176
28. Guinand B., Dujardin E., Dufour V. & Tsigenopoulos C. S. 2008. Characterisation of genetic structure of Dicentrarchus labrax larvae in two nurseries of the Gulf of Lions (NW Mediterranean). Aquatic Living Resources 21: 81-87
29. Chatziplis D., Batargias C., Tsigenopoulos C. S., Magoulas A., Kollias S., Kotoulas G., Volckaert F.A.M. and C.S. Haley 2007. Mapping Quantitative Trait Loci in European Sea Bass (Dicentrarchus labrax): The BASSMAP pilot study, Aquaculture, 272 Suppl. 1, S172-S182
30. Lymberakis P., Poulakakis N., Manthalou G., Tsigenopoulos C. S., Magoulas A. & Mylonas M. 2007. Mitochondrial phylogeography of Rana (Pelophylax) populations in the Eastern Mediterranean region. Molecular Phylogenetics and Evolution 44 (1): 115-125
31. Tsagkarakou A., Tsigenopoulos C. S., Gorman K., Lagnel J. & Bedford I. D. 2007. Biotype status and genetic polymorphism of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Greece: mitochondrial DNA and microsatellites. Bulletin of Entomological Research 97: 29-40
32. Franch R., Louro B., Tsalavouta M., Chatziplis D., Tsigenopoulos C. S., Sarropoulou E., Antonello J., Magoulas A., C. C. Mylonas, Patarnello T., Power D. M., Kotoulas G. & Bargelloni L. 2006. A genetic linkage map of the hermaphrodite teleost fish Sparus aurata L. Genetics 174: 851-861
33. Mantziou G., A. Antoniou, N. Poulakakis, G. Goulielmos, Tsigenopoulos C. S., Pinou T.& Mylonas M. 2005. Isolation and characterization of six polymorphic microsatellite markers in the fresh water turtle Mauremys rivulata (Testudines: Geoemydidae). Molecular Ecology Notes 5 (4): 727-729
34. Chistiakov D., Hellemans B., Haley C., Law A., Tsigenopoulos C. S., Kotoulas G., Bertotto D., Libertini A. & Volckaert F.A.M. 2005. A Microsatellite Linkage Map of the European Seabass Dicentrarchus labrax L. Genetics 170: 1821-1826
35. Poulakakis, N., Lymberakis, P., Tsigenopoulos, C. S., Magoulas, A. & Mylonas M. 2005. Phylogenetic relationships and evolutionary history of snaked-eyed skink Ablepharus kitaibelii (Sauria: Scincidae). Molecular Phylogenetics and Evolution) 34 (1): 245–256
36. Chistiakov D.A., Hellemans B., Tsigenopoulos C.S., Law A.S., Bartley N., Bertotto D., Libertini A., Kotoulas G., Haley C.S. & Volckaert F. A. M. 2004. Development and linkage relationships for new microsatellite markers of the sea bass (Dicentrarchus labrax L.). Animal Genetics 35 (1): 53-57
37. Tsigenopoulos C. S., Durand J. D., ünlü E. & Berrebi P. 2003. Rapid radiation of the Mediterranean Barbus species (Cyprinidae) after the Messinian salinity crisis of the Mediterranean Sea, inferred from mitochondrial phylogenetic analysis. Biological Journal of the Linnean Society 80 (2): 207-222
38. Tsigenopoulos C.S., Hellemans B., Chistiakov D.A., Libertini A., Kotoulas G. & Völckaert F. 2003. Eleven new microsatellites of the sea bass (Dicentrarchus labrax L.). Molecular Ecology Notes 3: 352-354
39. Berrebi P. & Tsigenopoulos C. S. 2003. Phylogenetic organization of the genus Barbus sensu stricto: A review based on data using molecular markers. “The Freshwater Fishes of Europe. Vol. 5/II: Cyprinidae, Part II: Barbus”, pp. 11-22, P. Banarescu and N. G. Bogutskaya Eds., AULA-Verlag
40. Kotlík P., Tsigenopoulos C. S., Ráb P. & Berrebi P. 2002. Two new Barbus species from the Danube River basin, with redescription of B. petenyi (Teleostei: Cyprinidae). Folia Zoologica 51 (3): 227-240
41. Tsigenopoulos C. S., Ráb P., Naran D. & Berrebi P. 2002. Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers. Heredity 88 (6): 466-473
42. Tsigenopoulos C. S., Kotlík P. & Berrebi P. 2002. Biogeography and pattern of gene flow among Barbus species (Teleostei: Cyprinidae) inhabiting the Italian Peninsula and neighboring Adriatic drainages as revealed by allozyme and mitochondrial sequence data. Biological Journal of the Linnean Society 75 (1): 83-99
43. Durand J. D., Tsigenopoulos C. S., ünlü E. & Berrebi P. 2002. Phylogeny and biogeography of the family Cyprinidae in the Middle East inferred from cytochrome b DNA – Evolutionary significance of this region. Molecular Phylogenetics and Evolution 22 (1): 91-100
44. Berrebi P. & Tsigenopoulos C. S. 2002. Contribution des séquences mitochondriales à l’éclatement phylogénétique du genre polyploïde Barbus (Téléostéen Cyprinidés). Implications biogéographiques. Biosystema 20: 49-56
45. Tsigenopoulos C. S. & Berrebi P. 2000. Molecular phylogeny of North Mediterranean freshwater barbs (genus Barbus: Cyprinidae) inferred from cytochrome b sequences: Biogeographic and systematic implications. Molecular Phylogenetics and Evolution 14 (2): 165-179
46. Tsigenopoulos C. S., Karakoysis Y. & Berrebi P. 1999. The North Mediterranean Barbus lineage: phylogenetic hypotheses and taxonomic implications based on allozyme data. Journal of Fish Biology 54 (2): 267-286
47. Tsingenopoulos C. S. & Karakousis Y. 1996. Phylogenetic relationships of Leuciscus keadicus, an endemic cyprinid species from Greece, with other species of the genus Leuciscus. Folia Zoologica 45: 87-93
2. Chaoui L., P-A.Gagnaire, B. Guinand, J-P. Quignard, C. Tsigenopoulos, M. H. Kara & F. Bonhomme 2012. Microsatellite length variation in candidate genes correlates with habitat in the gilthead sea bream Sparus aurata. Molecular Ecology, 21: 5497–5511
3. Loukovitis D., E. Sarropoulou, C. Batargias, A. P. Apostolidis, G. Kotoulas, C. S. Tsigenopoulos & D. Chatziplis 2012. Quantitative Trait Loci for Body Growth and Sex Determination in the Hermaphrodite Teleost Fish Sparus aurata L., Animal Genetics, 43: 753-759
4. Makridis P., Ferreira T., Kokou F., Tsigenopoulos C. & P. Divanach. 2012. Quantitative and Qualitative Aspects of Bacterial Communities Associated with Cultures of Chlorella minutissima. Journal of the World Aquaculture Society, 43: 571-578
5. Limborg M T, Hanel R, Debes P V, Ring AK, André C, Tsigenopoulos CS & D Bekkevold. 2012. Disentangling evolutionary processes shaping population structure across marine transition zones: Insights from a Pan European small pelagic fish. Heredity, 109: 96-107
6. Vogiatzi E., Hanel R., Dailianis T., Lagnel J., Hassan M., Magoulas A. & C.S Tsigenopoulos. 2012. Description of microsatellite markers in four mullids based on the development and cross-species amplification of 18 new markers in red mullet (Mullus barbatus). Biochemical Systematics and Ecology, 44: 279-285
7. Coscia I, Vogiatzi E, Kotoulas G, Tsigenopoulos C S. & S Mariani. 2012. Neutral and adaptive genetic variation in expanding populations of gilthead sea bream, Sparus aurata L., in the North-East Atlantic. Heredity, 108:537-546
8. Lefkaditou E., Tsigenopoulos C. S., Alidromiti C. & Haralabous J. 2012. On the occurrence of Alloteuthis subulata in the Eastern Ionian Sea and its distinction from the sympatric Alloteuthis media. Journal of Biological Research, 17: 169-175
9. Loukovitis D., Sarropoulou E., Vogiatzi E., Tsigenopoulos C.S., Kotoulas G., Magoulas A. & Chatziplis D. 2012 Genetic variation in farmed populations of the gilthead sea bream Sparus aurata in Greece using microsatellite DNA markers. Aquaculture Research, 43: 239-246
10. Rabaoui L., Mejri R., Bahri L., Tlig-Zouari S., Ben Hassine Oum Kalthoum & C. S. Tsigenopoulos. 2011 Genetic differentiation among populations of the endangered fan mussel Pinna nobilis (Mollusca) along the Tunisian coastline. Hydrobiologia, 678: 99-111
11. Dailianis T, Tsigenopoulos C, Dounas C & Voultsiadou E. 2011. Genetic diversity of the imperilled bath sponge Spongia officinalis Linnaeus, 1759 across the Mediterranean Sea: patterns of population differentiation and implications for taxonomy and conservation. Molecular Ecology, 20(18): 3757-3772
12. Boulton K, Massault C, Houston R, de Koning DJ, Haley C, Bovenhuis H, Batargias C, Canario AVM, Kotoulas G, C S Tsigenopoulos. 2011. QTL affecting morphometric traits and stress response in the gilthead seabream (Sparus aurata). Aquaculture, 319(1-2): 58-66
13. Vogiatzi E., Lagnel J., Pakaki V., Louro B., Canario A., Reinhardt R., Kotoulas G., Magoulas A. & C. S. Tsigenopoulos. 2011. In silico mining and characterization of simple sequence repeats from gilthead sea bream (Sparus aurata) expressed sequence tags (EST-SSRs); PCR amplification, polymorphism evaluation and multiplexing and cross-species assays. Marine Genomics, 4(2): 83-91
14. Hanel R. & Tsigenopoulos C.S. 2011. Phylogeny, Evolution and Taxonomy of sparids with some notes on their Ecology and Biology: Chapter 2. In “Sea bream: Biology & Aquaculture of Sparidae”, pp. 51-74, M. Pavlidis and C.C.Mylonas Eds, Wiley-Blackwell, ISBN 978-1-4051-9772-4
15. Loukovitis D., E. Sarropoulou, C. S. Tsigenopoulos, C. Batargias A. Magoulas, A. P. Apostolidis, D. Chatziplis & G. Kotoulas. 2011. Quantitative Trait Loci involved in sex determination and body growth in the gilthead sea bream (Sparus aurata L.) through targeted genome scan. PLoS ONE 6(1): e16599. doi:10.1371/journal.pone.0016599
16. Carvalho, G.R., Creer, S., Allen, M.J., Costa, F.O., Tsigenopoulos, C.S., Le Goff-Vitry, M., Magoulas, A., Medlin, L. & Metfies, K. 2010. Genomics in the Discovery and Monitoring of Marine Biodiversity, Chapter 1. In ‘Introduction to Marine Genomics’, pp. 1-32, Cock, J.M., Tessmar-Raible, K., Boyen, C. & Viard, F. Eds, Springer, ISBN 978-90-481-8616-7
17. Louro, B.E.P.., Passos, A.L.S., Souche E., Tsigenopoulos, C. , Beck, A., L., Bonhomme, F., Cancela, L., , Cerdà, J.6, Clark, M.S., Kotoulas, G., Lubzens, E., Planas, J., Volckaert, F.A.M., Reinhardt, R. & A.V.M Canario. 2010. Gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax) expressed sequence tags from multiple tissues: characterization, tissue specific expression and gene markers. Marine Genomics, 3(3-4): 179-191
18. Katharios P. & C.S. Tsigenopoulos. 2010. Nodavirus infection in cultured juvenile shi-drum, Umbrina cirrosa, in Greece. Aquaculture Research, 42: 147-152
19. Dailianis T. & Tsigenopoulos C. S. 2010. Characterization of polymorphic microsatellite markers for the endangered Mediterranean bath sponge Spongia offcinalis L. Conservation Genetics, 11: 1155-1158
20. Tsigenopoulos C. S., Kasapidis P. & Berrebi P. 2010. Phylogenetic relationships of hexaploid large-sized barbs (genus Labeobarbus, Cyprinidae) based on mtDNA data. Molecular Phylogenetics and Evolution 56(2): 851-856
21. Vogiatzi E., Kalogianni E., Giakoumi S., Magoulas A. & Tsigenopoulos C. S. 2009. Characterization of polymorphic microsatellite markers in Valencia letourneuxi (Valenciidae) and cross-amplification in two other cyprinodontiform species. Conservation Genetics Resources 1: 27-30
22. Lagnel J., Tsigenopoulos C. S. & Iliopoulos G. 2009. Noblast and Jamblast: new options and a java application manager for BLAST Bioinformatics 25(6):824-826
23. Volckaert F.A.M., Batargias C., Canario A., Chatziplis D., Chistiakov D., Haley C., Libertini A. & Tsigenopoulos C. S. 2008. European sea bass. In “Genome Mapping and Genomics in Fishes and Aquatic animals”, pp. 117-134. Thomas D. Kocher and Chittaranjan Kole Eds., Springer Verlag, ISBN 978-3-540-73836-7
24. Kyriazi P., N. Poulakakis, A. Parmakelis, P.A Crochet, J. Moravec, N. Rastegar-Pouyani, C. S. Tsigenopoulos, A. Magoulas, M. Mylonas & Lymberakis P. 2008. Mitochondrial DNA reveals the genealogical history of the Snake-eyed lizards (Ophisops elegans and O. occidentalis) (Sauria: Lacertidae). Molecular Phylogenetics and Evolution 49: 795–805
25. Chistiakov D.A., Tsigenopoulos C.S., Lagnel J., Guo Y.M., Hellemans B., Haley C.S., Volckaert F.A.M. & Kotoulas G. 2008. A combined AFLP and microsatellite linkage map and pilot comparative genomic analysis of European sea bass Dicentrarchus labrax L. Animal Genetics 39: 623-634
26. Dailianis, T., Limborg, M., Hanel, R., Bekkevold, D., Lagnel, J., Magoulas, A. and Tsigenopoulos, C. S. 2008 Characterization of nine polymorphic microsatellite markers in sprat (Sprattus sprattus L.). Molecular Ecology Resources 8: 861-863.
27. Apostolidis A., Loukovitis D. & Tsigenopoulos C. S. 2008. Genetic characterization of brown trout (Salmo trutta) populations from the Southern Balkans using mtDNA sequencing and RFLP analysis. Hydrobiologia 600: 169-176
28. Guinand B., Dujardin E., Dufour V. & Tsigenopoulos C. S. 2008. Characterisation of genetic structure of Dicentrarchus labrax larvae in two nurseries of the Gulf of Lions (NW Mediterranean). Aquatic Living Resources 21: 81-87
29. Chatziplis D., Batargias C., Tsigenopoulos C. S., Magoulas A., Kollias S., Kotoulas G., Volckaert F.A.M. and C.S. Haley 2007. Mapping Quantitative Trait Loci in European Sea Bass (Dicentrarchus labrax): The BASSMAP pilot study, Aquaculture, 272 Suppl. 1, S172-S182
30. Lymberakis P., Poulakakis N., Manthalou G., Tsigenopoulos C. S., Magoulas A. & Mylonas M. 2007. Mitochondrial phylogeography of Rana (Pelophylax) populations in the Eastern Mediterranean region. Molecular Phylogenetics and Evolution 44 (1): 115-125
31. Tsagkarakou A., Tsigenopoulos C. S., Gorman K., Lagnel J. & Bedford I. D. 2007. Biotype status and genetic polymorphism of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Greece: mitochondrial DNA and microsatellites. Bulletin of Entomological Research 97: 29-40
32. Franch R., Louro B., Tsalavouta M., Chatziplis D., Tsigenopoulos C. S., Sarropoulou E., Antonello J., Magoulas A., C. C. Mylonas, Patarnello T., Power D. M., Kotoulas G. & Bargelloni L. 2006. A genetic linkage map of the hermaphrodite teleost fish Sparus aurata L. Genetics 174: 851-861
33. Mantziou G., A. Antoniou, N. Poulakakis, G. Goulielmos, Tsigenopoulos C. S., Pinou T.& Mylonas M. 2005. Isolation and characterization of six polymorphic microsatellite markers in the fresh water turtle Mauremys rivulata (Testudines: Geoemydidae). Molecular Ecology Notes 5 (4): 727-729
34. Chistiakov D., Hellemans B., Haley C., Law A., Tsigenopoulos C. S., Kotoulas G., Bertotto D., Libertini A. & Volckaert F.A.M. 2005. A Microsatellite Linkage Map of the European Seabass Dicentrarchus labrax L. Genetics 170: 1821-1826
35. Poulakakis, N., Lymberakis, P., Tsigenopoulos, C. S., Magoulas, A. & Mylonas M. 2005. Phylogenetic relationships and evolutionary history of snaked-eyed skink Ablepharus kitaibelii (Sauria: Scincidae). Molecular Phylogenetics and Evolution) 34 (1): 245–256
36. Chistiakov D.A., Hellemans B., Tsigenopoulos C.S., Law A.S., Bartley N., Bertotto D., Libertini A., Kotoulas G., Haley C.S. & Volckaert F. A. M. 2004. Development and linkage relationships for new microsatellite markers of the sea bass (Dicentrarchus labrax L.). Animal Genetics 35 (1): 53-57
37. Tsigenopoulos C. S., Durand J. D., ünlü E. & Berrebi P. 2003. Rapid radiation of the Mediterranean Barbus species (Cyprinidae) after the Messinian salinity crisis of the Mediterranean Sea, inferred from mitochondrial phylogenetic analysis. Biological Journal of the Linnean Society 80 (2): 207-222
38. Tsigenopoulos C.S., Hellemans B., Chistiakov D.A., Libertini A., Kotoulas G. & Völckaert F. 2003. Eleven new microsatellites of the sea bass (Dicentrarchus labrax L.). Molecular Ecology Notes 3: 352-354
39. Berrebi P. & Tsigenopoulos C. S. 2003. Phylogenetic organization of the genus Barbus sensu stricto: A review based on data using molecular markers. “The Freshwater Fishes of Europe. Vol. 5/II: Cyprinidae, Part II: Barbus”, pp. 11-22, P. Banarescu and N. G. Bogutskaya Eds., AULA-Verlag
40. Kotlík P., Tsigenopoulos C. S., Ráb P. & Berrebi P. 2002. Two new Barbus species from the Danube River basin, with redescription of B. petenyi (Teleostei: Cyprinidae). Folia Zoologica 51 (3): 227-240
41. Tsigenopoulos C. S., Ráb P., Naran D. & Berrebi P. 2002. Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers. Heredity 88 (6): 466-473
42. Tsigenopoulos C. S., Kotlík P. & Berrebi P. 2002. Biogeography and pattern of gene flow among Barbus species (Teleostei: Cyprinidae) inhabiting the Italian Peninsula and neighboring Adriatic drainages as revealed by allozyme and mitochondrial sequence data. Biological Journal of the Linnean Society 75 (1): 83-99
43. Durand J. D., Tsigenopoulos C. S., ünlü E. & Berrebi P. 2002. Phylogeny and biogeography of the family Cyprinidae in the Middle East inferred from cytochrome b DNA – Evolutionary significance of this region. Molecular Phylogenetics and Evolution 22 (1): 91-100
44. Berrebi P. & Tsigenopoulos C. S. 2002. Contribution des séquences mitochondriales à l’éclatement phylogénétique du genre polyploïde Barbus (Téléostéen Cyprinidés). Implications biogéographiques. Biosystema 20: 49-56
45. Tsigenopoulos C. S. & Berrebi P. 2000. Molecular phylogeny of North Mediterranean freshwater barbs (genus Barbus: Cyprinidae) inferred from cytochrome b sequences: Biogeographic and systematic implications. Molecular Phylogenetics and Evolution 14 (2): 165-179
46. Tsigenopoulos C. S., Karakoysis Y. & Berrebi P. 1999. The North Mediterranean Barbus lineage: phylogenetic hypotheses and taxonomic implications based on allozyme data. Journal of Fish Biology 54 (2): 267-286
47. Tsingenopoulos C. S. & Karakousis Y. 1996. Phylogenetic relationships of Leuciscus keadicus, an endemic cyprinid species from Greece, with other species of the genus Leuciscus. Folia Zoologica 45: 87-93
