Mylonas Constantinos (Dinos) C.

by / Friday, 14 November 2014 / Published in people



Position: Research Director

Contact Info

Institute: Aquaculture
Address: Thalassocosmos, Aqualabs P.O. Box 2214, Iraklion, Crete 71003 Greece
Tel: +302810337878
Telex: +302810337778


Dr. C.C. Mylonas’ involvement in the field of aquaculture started with his undergraduate studies in Aquaculture (B.Sc.), with the intention of finding employment in the emerging marine aquaculture industry in the Mediterranean in the late 1980s. With the decision of continuing his education, he studied the application of pharmacological methods for the induction of ovulation in salmonids and the effect of the treatment on egg quality, through the pursuit of a M.Sc. degree under Drs. Craig V. Sullivan and Jeffrey Hinshaw. Remaining in the academia (Ph.D.), he studied the reproductive endocrinology of fish and the use of controlled-release delivery systems for gonadotropin-releasing hormone agonist (GnRHa), in the induction of ovulation and enhancement of sperm production in various cultured fishes, under the supervision of Professor Yonathan Zohar.

During his tenure as a researcher at the Institute of Aquaculture, HCMR, Dr. C.C. Mylonas has been involved in four EU-funded projects (5th and 7th FP), and 15 National or Binational research programmes in the area of fish reproduction, broodstock management, gamete quality improvement and species diversification. One of his major recent achievements was the control of reproduction of Atlantic bluefin tuna in captivity, and the production of viable eggs (Mylonas, C.C. et al. 2007. Preparation and administration of gonadotropin-releasing hormone agonist (GnRHa) implants for the artificial control of reproductive maturation in captive-reared Atlantic bluefin tuna (Thunnus thynnus thynnus). Reviews in Fisheries Science 15: 183-210). The success of that work was followed, recently, with the production of Atlantic bluefin tuna fingerlings and their stocking in grow-out cages.

In addition to research, Dr. C.C. Mylonas is involved in teaching graduate students and professionals in the area of fish reproduction, broodstock management and assisted reproduction technologies, through international courses (Instituto Agronomico Mediterraneo de Zaragoza, Spain) and seminars at various European Universities. He is also involved in training graduate students through research undertaken under his supervision at the Institute of Aquaculture, HCMR.

Dr. C.C. Mylonas is also very active in consulting with the European aquaculture industry and provides assistance with broodstock management, spawning induction methods and gamete quality evaluations, for various cultured fishes.

Dr. Mylonas has authored 83 articles in international ISI journals, five chapters in multi-authored books and has recently edited a book on Sparidae aquaculture (Pavlidis, M., Mylonas, C.C., 2011. Sparidae: biology and aquaculture of gilthead sea bream and related species. Blackwell Scientific Publishers, London, 390 pp.)

Educational Profile

Ph.D., Marine-Estuarine and Environmental Sciences, Center of Marine Biotechnology, University of Maryland, USA, 1996
M.Sc., Zoology, North Carolina State University, USA, 1991
B.Sc., (Summa Cum Laude Honors), Wildlife and Fisheries Sciences, Texas A&M University, USA, 1988

Professional Profile

2007-present: Director of Research, Institute of Aquaculture, Hellenic Center for Marine Research, Greece.
2002-2007: Level B Researcher, Institute of Aquaculture, Hellenic Center for Marine Research, Greece.
1999-2002: Level C Researcher, Institute of Marine Biology of Crete, Greece.
1998-1999: Post-Doctoral Research Associate, Center of Marine Biotechnology, University of Maryland Biotechnology Institute, USA
1996-1998: Assistant Hatchery Manager, Sagro Aquaculture Ltd., Cyprus.

Scientific and Professional Memberships

World Aquaculture Society
European Aquaculture Society
The Fisheries Society of the British Isles
Editorial Board Member of the journal “Aquaculture”
Editorial Board Member of the “Israeli Journal of Aquaculture-Bamidgeh”

Awards and Recognition

1985-1988: Full undergraduate studies scholarship from USA-AMIDEAST
1993: Best Poster Presentation Award, Aquaculture Europe 1993, European Aquaculture Society

Research Interests

Dr. C.C. Mylonas’ research field includes (a) the study of fish reproductive biology and endocrinology, (b) the identification of the dysfunctions exhibited by female and male broodstocks in captive conditions, and (c) the development of pharmacological methods for the control of reproduction, induction of spawning and improvement of sperm production in fish of interest for the aquaculture industry. In this framework, Dr. C.C. Mylonas’ has been involved in basic and, mainly, applied research, aiming at the optimization of broodstock management methods, focusing on marine fishes. Therefore, in addition to his cooperation with various research centers, his research put him in close contact with the aquaculture industry, and has studied more than twenty different cultured fish species.
In the last few years, Dr. C.C. Mylonas’ research focuses on the study of large benthic and pelagic fishes such as the wreckfish (Polyprion americanus), greater amberjack (Seriola dumerili) and the Atlantic bluefin tuna (Thunnus thynnus), species which he succeeded in controlling their reproduction in captivity for the first time, world-wide. These successes have opened a new chapter in the potential of marine aquaculture, not only in Greece and Europe, but world wide, since all three species are cosmopolitan in distribution and highly demanded in the seafood markets.
In the future, Dr. C.C. Mylonas intents to continue working in the study of reproductive biology of fishes, and the problems they present in the aquaculture industry, and in the optimization of broodstock management methods of already cultured, as well as new species with potential for the aquaculture industry. Even in the species that reproduce spontaneously after many years of culture, there is still a need for improvement of the production and gamete quality, as well as in the development of artificial insemination methods, in order to be able to undertake genetic improvement programs and cryopreservation of gametes from selected stocks.

1. Mylonas, C.C., Hinshaw, J.M. and Sullivan, C.V. 1992. GnRHa-induced ovulation of brown trout (Salmo trutta) and its effects on egg quality. Aquaculture, 106: 379-392.
2. Mylonas, C.C., Sullivan, C.V. and Hinshaw, J.M. 1994. Thyroid hormones in brown trout (Salmo trutta) reproduction and early development. Fish Physiology and Biochemistry, 13: 485-493.
3. Mylonas, C., Zohar, Y., Richardson, B.M., Minkkinen, S.P. 1995. Induced spawning of wild American shad (Alosa sapidissima) using sustained administration of gonadotropin-releasing hormone analog (GnRHa). Journal of the World Aquaculture Society, 26: 240-251.
4. Mylonas, C.C., Tabata, Y., Langer, R., Zohar, Y. 1995. Preparation and evaluation of polyanhydride microspheres containing gonadotropin-releasing hormone (GnRH), for inducing ovulation and spermiation in fish. Journal of Controlled Release, 35: 23-34.
5. Mylonas, C.C., Magnus, Y., Gissis, A., Klebanov, Y. and Zohar, Y. 1996. Application of controlled-release, GnRHa-delivery systems in commercial production of white bass x striped bass hybrids (sunshine bass), using captive broodstocks. Aquaculture, 140: 265-280.
6. Holland, M.C., Mylonas, C.C. and Y. Zohar. 1996. Sperm characteristics of precocious 1-year-old male striped bass, Morone saxatilis. Journal of the World Aquaculture Society. 27: 208-212.
7. Sorbera, L.A., Mylonas, C.C., Zanuy, S., Carrillo, M. and Zohar, Y. 1996. Sustained administration of GnRHa increases sperm volume without altering sperm counts in the sea bass. The Journal of Experimental Zoology, 276: 361-368.
8. Mylonas, C.C., Woods, L.C. III, and Y. Zohar. 1997. Cyto-histological examination of post-vitellogenesis and final oocyte maturation in captive-reared striped bass. Journal of Fish Biology, 50: 34-49.
9. Mylonas, C.C., Gissis, A., Magnus, Y. and Y. Zohar. 1997. Hormonal changes in male white bass (Morone chrysops) and evaluation of milt quality after treatment with a sustained-release GnRHa-delivery system. Aquaculture, 153: 301-311.
10. Mylonas, C.C., Magnus, Y., Klebanov, Y., Gissis, A., and Y. Zohar. 1997. Reproductive biology and endocrine regulation of final oocyte maturation of captive white bass. Journal of Fish Biology, 51: 234-250.
11. Mylonas, C.C., Scott, A.P., Vermeirssen, E.L.M. and Y. Zohar. 1997. Changes in plasma gonadotropin II and sex steroid hormones, and sperm production of striped bass after treatment with controlled-release gonadotropin-releasing hormone agonist-delivery systems. Biology of Reproduction, 57: 669-675.
12. Mylonas, C.C., Scott, A.P. and Y. Zohar. 1997. Plasma gonadotropin II, sex steroids, and thyroid hormones in wild striped bass (Morone saxatilis) during spermiation and final oocyte maturation. General and Comparative Endocrinology, 108: 223-236.
13. Schultz, R.W., van der Wind, F., Janssen-Dommerholt, C., Peute, J., Mylonas, C.C., Zohar, Y., Swanson, P., and H.J.Th. Goos. 1997. Modulation of testicular androgen production in adolescent African catfish (Clarias gariepinus). General and Comparative Endocrinology, 108: 56-66.
14. Larsson, D.G.J., Mylonas, C.C., Zohar, Y. and L.W. Crim. 1997. Gonadotropin releasing hormone-analogue (GnRH-A) induces multiple ovulations of high-quality eggs in a cold-water, batch-spawning teleost, the yellowtail flounder (Pleuronectes ferrugineus). Canadian Journal of Fisheries and Aquatic Sciences, 54: 1957-1964.
15. Mylonas, C.C., Woods, L.C. III, Thomas, P. and Y. Zohar. 1998. Endocrine profiles of female striped bass (Morone saxatilis) in captivity, during post-vitellogenesis and induction of final oocyte maturation via controlled-release GnRHa-delivery systems. General and Comparative Endocrinology, 110: 276-289.
16. Vermeirssen, E.L.M., Scott, A.P., Mylonas, C.C. and Y. Zohar. 1998. Gonadotrophin-releasing hormone agonist stimulates milt fluidity and plasma concentrations of 17,20?-dihydroxylated and 5?-reduced, 3?-hydroxylated C21 steroids in male plaice (Pleuronectes platessa). General and Comparative Endocrinology, 112: 163-177.
17. Mylonas, C.C., Zohar, Y., Woods, L.C. III, Thomas, P. and R. W. Schulz. 1998. Hormone profiles of captive striped bass Morone saxatilis during spermiation, and long-term enhancement of milt production. Journal of the World Aquaculture Society, 29: 379-392.
18. Greenwood, L.N., Scott, A.P., Vermeirssen, E.L.M., Mylonas, C.C. and M. Pavlidis. 2001. Plasma steroids in mature common dentex (Dentex dentex) stimulated with a gonadotropin-releasing hormone agonist. General and Comparative Endocrinology, 123: 1-12.
19. Zohar, Y. and C.C. Mylonas. 2001. Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture 197: 99-136.
20. Mylonas. C.C. and Y. Zohar. 2001. Use of GnRHa-delivery systems for the control of reproduction in fish. Reviews in Fish Biology and Fisheries, 10: 463-491.
21. Fornies, M.A., Mananos, E., Carrillo, M., Rocha, A., Laureau, S., Mylonas, C.C., Zohar, Y. and S. Zanuy. 2001. Spawning induction of individual European sea bass females (Dicentrarchus labrax) using different GnRHa-delivery systems. Aquaculture, 202: 221-234.
22. Panini, E., Mylonas, C.C., Zanuy, S., Carrillo M., Ramos, J. and M.P. Bruce. 2002. Incubation of embryos and larvae of marine fish using microtiter plates. Aquaculture International, 9: 189-195.
23. Mylonas, C.C. and Y. Zohar. 2001. Endocrine regulation and artificial induction of oocyte maturation and spermiation in basses of the genus Morone. Aquaculture, 202: 205-220.
24. Mananos, E., Carrillo, M., Sorbera, L.A., Mylonas, C.C., Asturiano, J.F., Bayarri, M.J., Zohar, Y. and S. Zanuy. 2002. Luteinizing hormone and sexual steroid plasma levels after treatment of European sea bass with sustained-release delivery systems for gonadotropin-releasing hormone analogue. Journal of Fish Biology, 60: 328-339.
25. Rainis, S. Mylonas, C.C., Kyriakou, Y. and P. Divanach. 2003. Enhancement of spermiation in European sea bass (Dicentrarchus labrax) at the end of the reproductive season using GnRHa implants. Aquaculture, 219: 873-890.
26. Marino, G, Panini, E., Longobardi, A., Mandich, A., Finoia, M.G., Zohar, Y. and C.C. Mylonas. 2003. Induction of ovulation in captive-reared dusky grouper, Epinephelus marginatus (Lowe, 1834) with a sustained-release GnRHa implant. Aquaculture, 219: 841-858.
27. Mylonas C.C., Sigelaki, I., Divanach, P., Mananos, E., Carillo, M. and A. Afonso-Polyviou. 2003. Multiple spawning and egg quality of individual European sea bass (Dicentrarchus labrax) females after repeated injections of GnRHa. Aquaculture, 221: 605-620.
28. Mylonas, C.C., Papadaki, M. and P. Divanach. 2003. Seasonal changes in sperm production and quality in the red porgy Pagrus pagrus (L.). Aquaculture Research, 34: 1161-1170.
29. Mylonas, C.C., Anezaki, L., Divanach, P., Zanuy, S., Piferrer, F., Ron, B., Peduel, A., Ben Atia, I., Gorshkov, S.and A. Tandler. 2003. Influence of rearing temperature at two periods during early life on growth and sex differentiation of two strains of European sea bass. Fish Physiology and Biochemistry, 28: 167-168.
30. Rodriguez, L., Halm, S., Mylonas, C. and S. Zanuy. 2003. Gene expression of estrogen receptors ? and ? during early sexual differentiation in the European sea bass (Dicentrarchus labrax). Fish Physiology and Biochemistry, 28: 175.
31. Mylonas, C.C., Papadaki, M., Pavlidis, M. and P. Divanach. 2004. Evaluation of egg production and quality in the Mediterranean red porgy (Pagrus pagrus) during two consecutive spawning seasons. Aquaculture, 232: 637-649.
32. Mylonas, C.C., Kyriakou, Y., Sigelaki, I, Georgiou, G., Stephanou, D. and P. Divanach. 2004. Reproductive biology of the shi drum (Umbrina cirrosa) in captivity and induction of spawning using GnRHa. Israeli Journal of Aquaculture-Bamidgeh, 56: 75-92.
33. Mylonas, C.C., Papandroulakis, N., Smboukis, A., Papadaki, M. and P. Divanach. 2004. Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants. Aquaculture, 237: 141-154.
34. Halm, S., Martinez-Rodriguez, G., Rodriguez, L., Prat, F., Mylonas, C.C., Carrillo, M. and S. Zanuy. 2004. Cloning, characterisation, and expression of three oestrogen receptors (ERa, ERb1 and ERb2) in the European sea bass, Dicentrarchus labrax. Molecular and Cellular Endocrinology, 223: 63-75.
35. Galay-Burgos, M., Llewellyn, L., Mylonas, C.C., Canario, A.V.M., Zanuy, S. and G.E. Sweeney. 2004. Analysis of Sox gene family in the European sea bass (Dicentrarchus labrax). Comparative Biochemistry and Physiology, B137: 279-284.
36. Papadaki, M., Piferrer, F., Zanuy, S., Maingot, E., Divanach, P. and C.C. Mylonas. 2005. Growth, sex differentiation and gonad and plasma levels of sex steroids in male- and female-dominant populations of Dicentrarchus labrax obtained through repeated size grading. Journal of Fish Biology, 66: 938-956.
37. Papandroulakis, N., Mylonas, C.C., Maingot, E. and P. Divanach. 2005. First results of greater amberjack (Seriola dumerili) larval rearing in mesocosm. Aquaculture, 250: 155-161.
38. Mylonas, C.C., Cardinaletti, G., Sigelaki, I. and A. Polzonetti-Magni. 2005. Comparative efficacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) at different temperatures. Aquaculture, 246: 467-481.
39. Mylonas, C.C., Anezaki, L., Divanach, P., Zanuy, S., Piferrer, F., Ron, B., Peduel, A., Ben Atia, I., Gorshkov, S. and A. Tandler. 2005. Influence of rearing temperature during the larval and nursery periods on growth and sex differentiation in two Mediterranean strains of Dicentrarchus labrax. Journal of Fish Biology, 67: 652-668.
40. Koumoundouros, G. Kouttouki, S., Georgakopoulou, E., Papadakis, I, Maingot, E., Kaspiris, P., Kiriakou, Y., Georgiou, G., Divanach, P., Kentouri, M. and C.C. Mylonas. 2005. Ontogeny of the shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture. Aquaculture Research, 36: 1265-1272.
41. Kokokiris, L., Canario, A.V.M., Mylonas C., Pavlidis, M., Kentouri, M. and P. Divanach. 2005. Induction of ovulation and spawning in the Mediterranean red porgy porgy, Pagrus pagrus, by controlled delivery and acute injection of GnRHa. Israeli Journal of Aquaculrure-Bamidgeh, 57: 223-230.
42. Sarter, K., Papadaki, M., Zanuy, S. and C.C. Mylonas. 2006. Permanent sex inversion of 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17?-methyltesosterone implants. Aquaculture, 256: 443-456.
43. Agulleiro, M.J., Anguis, V., Canavate, J.P., Martinez-Rodriguez, G., Mylonas, C.C. and J. Cerda. 2006. Induction of spawning of captive-reared Senegal sole (Solea senegalensis) using different administration methods for gonadotropin-releasing hormone agonist. Aquaculture, 257: 511-524.
45. Franch, R., Louro, B., Tsalavouta, M., Chatziplis, D., Tsigenopoulos, C.S., Sarropoulou, E., Antonello, J., Magoulas, A., Mylonas, C.C., Babbucci, M., Patarnello, T., Power, D.M., Kotoulas, G. and L. Bargelloni. 2006. A genetic linkage map of the hermaphrodite teleost fish Sparus aurata L. Genetics, 174: 851-861.
46. Moles, G., Carrillo, M., Mananos, E., Mylonas, C.C. and S. Zanuy. 2007. Temporal profile of brain and pituitary GnRHs, GnRH-R and gonadotropin mRNA expression and content during early development in European sea bass (Dicentrarchus labrax L.). General and Comparative Endocrinology, 150: 75-86.
47. Socorro, S., Martins, R.S, Deloffre, L., Mylonas, C.C. and A.V.M. Canario. 2007. A cDNA for European sea bass (Dicentrarchus labrax) 11?-hydroxylase: gene expression during the thermosensitive period and gonadogenesis. General and Comparative Endocrinology, 150: 164-173.
48. Katharios, P., Agathaggelou A., Paraskevopoulos, S. and C.C. Mylonas. 2007. Comparison of Iodine and gluteraldehyde as surface disinfectants for red porgy (Pagrus pagrus) and white sea bream (Diplodus sargus sargus) eggs. Aquaculture Research, 38: 527-536.
49. Katharios, P., Garaffo, M., Sarter, K., Athanassopoulou, F. and C.C. Mylonas. 2007. A case of high mortality due to heavy infestation of Ceratomyxa diplodae in sharpsnout sea bream (Diplodus puntazzo) treated with reproductive steroids. Bulletin of the European Association of Fish Pathologists, 27: 43-47.
50. Agulleiro, M.J., Scott, A.P., Duncan, N., Mylonas, C.C. and J. Cerda. 2007. Treatment of GnRHa-implanted Senegalese sole (Solea senegalensis) with 11-ketoandrostenedione stimulates spermatogenesis and increases sperm motility. Comparative Biochemistry and Physiology A147: 885-892.
51. Martins, R.S., Deloffre, L., Mylonas, C.C., Power, D.M. and A.V.M. Canario. 2007. Developmental expression of DAX1 in the European sea bass, Dicentrarchus labrax: lack of evidence for sexual dimorphism during sex differentiation. Reproductive Biology and Endocrinology 5:19, doi:10.1186/1477-7827-5-19.
52. Mylonas, C.C., Bridges, C., Gordin, H., Belmonte Rios, A., Garcia, A., De la Gandara, F., Fauvel, C., Suquet, M., Medina, A., Papadaki, M., Heinisch, G., De Metrio, G., Corriero, A., Vassallo-Agius, R., Guzman, J.M., Mananos, E. and Y. Zohar. 2007. Preparation and administration of gonadotropin-releasing hormone agonist (GnRHa) implants for the artificial control of reproductive maturation in captive-reared Atlantic bluefin tuna (Thunnus thynnus thynnus). Reviews in Fisheries Science 15: 183-210.
53. Corriero, A., Medina, A., Mylonas, C.C., Abascal, F.J., Deflorio, M., Aragon, L., Bridges, C.R., Santamaria, N., Heinisch, G., Vassallo-Agius, R., Belmonte, A., Fauvel, C., Garcia, A., Gordin, H. and G. De Metrio. 2007. Histological study of the effects of treatment with gonadotropin-releasing hormone agonist (GnRHa) on the reproductive maturation of captive-reared Atlantic bluefin tuna (Thunnus thynnus L.). Aquaculture, 272: 675-686.
54. M. Papadaki, M. Papadopoulou, I. Siggelaki and C.C. Mylonas. 2008. Egg and sperm production and quality of sharpsnout sea bream (Diplodus puntazzo) in captivity. Aquaculture 276: 187-197.
55. Guzman, J.M., Norberg, B., Ramos, J., Mylonas, C.C. and E. Mananos. 2008. Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis). General and Comparative Endocrinology 156: 285-297.
56. Heinisch, G., Corral, N., Medina, A., Abascal, F.J., De la Serna, J.M., Vassallo-Agius, R., Belmonte Rios, A., Garcia, A., De la Gandara, F., Fauvel, C., Bridges, C.R., Mylonas, C.C., Karakulak, F.S., Oray, I.K., De Metrio, G., Rosenfeld, H. and H. Gordin. 2008. Spatial-temporal pattern of bluefin tuna (Thunnus thynnus L. 1758) gonad maturation across the Mediterranean Sea. Marine Biology 154: 623-630.
57. Marco-Jimenez, F., Penaranda, D.S., Perez, L., Viudes-de-Castro, M.P., Mylonas, C.C., Jover, M. and J.F. Asturiano. 2008. Morphometric characterization of sharpsnout sea bream (Diplodus puntazzo) and gilthead sea bream (Sparus aurata) spermatozoa using computer-assisted spermatozoa analysis (ASMA). Journal of Applied Ichthyology 24: 382-385.
58. Penaranda, D.S., Perez, L., Fakriadis, G., Mylonas, C.C. and J.F. Asturiano. 2008. Effects of extenders and cryoprotectant combinations on motility and morphometry of sea bass (Dicentrarchus labrax) spermatozoa. Journal of Applied Ichthyology 24: 450-455.
59. Blazquez, M., Gonzalez, A., Papadaki, M., Mylonas, C. and F. Piferrer. 2008. Sex-related changes in estrogen receptors and aromatase gene expression and enzymatic activity during early development and sex differentiation in the European sea bass (Dicentrarchus labrax). General and Comparative Endocrinology 158: 95-101.
60. Micale, V., Di Giancamillo, A., Domeneghini, C., Mylonas, C.C., Nomikos, N., Papadakis, I.E. and U. Muglia. 2008. Ontogeny of the digestive tract in sharpsnout sea bream Diplodus puntazzo (Cetti, 1777). Histology and Histopathology 23: 1077-1091.
61. Fauvel, C., Suquet, M., Severe, A., Mylonas, C.C. and N. Papandroulakis. 2008. Slow-release GnRHa therapy prevented atresia during vitellogenesis and induced ovulation of captive wreckfish (Polyprion americanus). Cybium 32(2) suppl.: 191.
62. Duncan, N., Estevez, A., Padros, F., Aguillera, C., Montero, F.E., Norambuena, F., Carazo, I., Carbo, R. and C.C. Mylonas. 2008. Acclimation to captivity and GnRHa-induced spawning of meagre (Argyrosomus regius). Cybium 32(2) suppl.: 332-333.
63. Fanouraki, E., Papandroulakis, N., Ellis, T., Mylonas C.C., Scott, A.P. and M. Pavlides. 2008. Water cortisol is a reliable indicator of stress in European sea bass, Dicentrarchus labrax. Behaviour 145: 1267-1281.
64. Papadakis, I.E., Zaiss, M., Maingot, E., Divanach, P., Kyriakou, Y., Georgiou, G. and C.C. Mylonas. 2009. Histological comparison of the digestive tract ontogeny in shi drum (Umbrina cirrosa L.) fed with or without Artemia. Aquaculture 286: 45-52.
65. Mylonas, C.C., Pavlidis, M., Papandroulakis, N., Zaiss, M., Tsafarakis, D., Papadakis, I.E. and S. Varsamos. 2009. Growth performance and osmoregulation in the shi drum (Umbrina cirrosa L.) adapted to different environmental salinities. Aquaculture 287: 203-210.
66. Corriero A., Medina A., Mylonas C.C., Bridges C.R., Santamaria N., Deflorio M., Losurdo M., Zupa R., Gordin H., de la Gandara F., Belmonte Rios A., Pousis C., De Metrio G. 2009. Proliferation and apoptosis of male germ cells in captive Atlantic bluefin tuna (Thunnus thynnus L.) treated with gonadotropin-releasing hormone agonist (GnRHa). Animal Reproduction Science 116: 346-357.
67. Guzman, J.M., Ramos, J., Mylonas, C.C., Mananos, E., 2009. Spawning performance and plasma levels of GnRHa and sex steroids in cultured female Senegalese sole (Solea senegalensis) treated with different GnRHa-delivery systems. Aquaculture 291: 200-209.
68. Deloffre, L., Martins, R.S.T., Mylonas, C.C., Canario, A.V.M., 2009. Alternative transcripts of DMRT 1 in the European sea bass: expression during gonadal differentiation. Aquaculture 293: 89-99.
69. Mylonas, C.C., Fostier, A., Zanuy, S., 2010. Broodstock management and hormonal manipulations of reproduction. General and Comparative Endocrinology 165: 516-534.
70. De Metrio, G., Bridges, C.R., Mylonas, C.C., Caggiano, M., Deflorio, M., Santamaria, N., Zupa, P., Pousis, C., Vassallo-Agius, R., Gordin, H., Corriero, A., 2010. Spawning induction and large-scale collection of fertilized eggs in captive Atlantic bluefin tuna (Thunnus thynnus L.) and the first larval rearing efforts. Journal of Applied Ichthyology 26: 596-599.
71. Chatzifotis, S., Panagiotidou, M., Papaioannou, N., Pavlidis, M., Nengas, I., Mylonas, C.C., 2010. Effect of dietary lipid levels on growth, feed utilization, body composition and serum metabolites of meagre (Argyrosomus regius) juveniles. Aquaculture 307: 65-70.
72. Mylonas, C.C., Corriero, A., De la Gandara, F., Belmonte Rios, A., 2010. Atlantic bluefin tuna (Thunnus thynnus) farming and fattening in the Mediterranean Sea. Reviews in Fisheries Sciences 18: 266-280.
73. Suquet, M., Cosson, J., de la Gandara, F., Mylonas, C.C., Papadaki, M., Lallemant, S., Fauvel, C., 2010. Sperm features of captive Atlantic bluefin tuna (Thunnus thynnus). Journal of Applied Ichthyology 26: 775-778.
74. Pousis, C., De Giorgi, C., Mylonas, C.C., Bridges, C.R., Zupa, P., Vassallo-Agius, R., de la Gandara, F., Dileo, C., De Metrio, G., Corriero, A., 2011 Comparatice study of liver vitellogenin gene expression and oocyte yolk accumulation in wild and captive Atlantic bluefin tuna (Thunnus thynnus L.). Animal Reproduction Science 123: 98-105.
75. Blazquez, M., Gonzalez, A., Mylonas, C.C., Piferrer, F., 2011 Cloning and sequence analysis of a vasa homolog in the European sea bass (Dicentrarchus labrax): tissue distribution and mRNA expression levels during early development and sex differentiation. Gen. Comp. Endocrinol. 170: 322-333.
76. Guzman, J.M., Cal, R., Garcia-Lopez, A., Chereguini, O., Kight, K.E., Olmedo, M., Sarasquete, C., Mylonas, C.C., Peleteiro, J.B., Zohar, Y., Mananos, E., 2011. Effects of in vivo treatment with the dopamine antagonist pimozide and gonadotropin-releasing hormone agonist (GnRHa) on the reproductive axis of Senegalese sole (Solea senegalensis). Comp. Biochem. Physiol. 158: 235-245.
77. Guzman, J.M., Ramos, J., Mylonas, C.C., Mananos, E., 2011. Comparative effects of human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone agonist (GnRHa) treatments on the stimulation of male Senegalese sole (Solea senegalensis) reproduction. Aquaculture 316: 121-128.
78. Corriero, A., Zupa, P., Bello, G., Mylonas, C.C., Santamaria, C.A., Deflorio, M., Genovese, S., Basilone, G., Buscaino, G., Buffa, G., Pousis, C., De Metrio, G., 2011. Evidence that severe acute stress and starvatoin induce rapid atresia of ovarian vitellogenic follicles in Atlantic bluefin tuna, Thunnus thynnus (Osteichthyes: Scombridae). Journal of Fish Diseases 000: 000-000. (in press)
79. Fanouraki, E., Mylonas, C.C., Papandroulakis, N., Pavlidis, M., 2011. Species specificity in the magnitude and duration of the acute stress response in Mediterranean marine fish in culture. Gen. Comp. Endocrinol. 000: 000-000. (in press)
80. Aranda, G., Aragon, L., Corriero, A., Mylonas, C.C., De la Gandara, F., Belmonte Rios, A., Medina, A., 2011. GnRHa-induced spawning in cage-reared bluefin tuna: An evaluation using stereological quantification of ovarian post-ovulatory follicles. Aquaculture 000: 000-000. (in press)
81. Sfakianakis, D.G., Leris, I., Mylonas, C.C., Kentouri, M., 2011. Temperature during early life determines sex in zebrafish, Danio rerio (Hamilton, 1822). Journal of Biological Research 000: 000-000. (in press)
82. Moles, G., Gomez, A., Carrillo, M., Rocha, A., Mylonas, C.C., Zanuy, S., 2011. Determination of Fsh quantity and bioactivity during sex differentiation and oogenesis in European sea bass using a newly developed immunoassay and an in vitro bioassay. Biol. Reprod. 000: 000-000. (in press)
83. Vazirzadeh, A., Mojazi Amiri, B., Yelghi, S., Hajimoradloo, A., Nematollahi, M.A., Mylonas, C.C., 2011. Comparison of the effects of different methods of mammalian and salmon GnRHa administration on spawning performance in wild-caught female carp (Cyprinus carpio carpio) from the Caspian Sea. Aquaculture 320: 123-128.

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