Position: Scientific research technician
Institute: Marine Biology and Genetics
Address: HCMR, Gournes Pediados P.O.Box 2214, Irakleion 71003, Crete, Greece.
He has a long time experience in molecular biology techniques and protocols commonly used in population genetic studies and selective breeding programs.
Stelios Darivianakis holds a bachelor’s degree in Chemistry from Aristotle University, Thessaloniki, Greece.
â€¢ Graduated from Lyceum, Crete 1983.
â€¢ B.Sc. in Chemistry, Aristotle University, Thessaloniki, Greece.
â€¢ 1996-1997: Laboratory technician at the Department of Biology – University of Crete.
â€¢ 1997-to date: Scientific research technician at the Department of Genetics and Molecular Biotechnology of the Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research.
Specific Training and Skills
Biochemical and Molecular Biology protocols, experience in laboratory research techniques i.e. DNA extraction, protein electrophoresis, Polymerase Chain Reaction (PCR), RFLPs, AFPLs, Genotyping, Sequencing, Genetic DNA Analyzer (exclusive user) and next-generation library preparation (ABI 3730, GS-FLX 454), microsatellite data analysis etc. Morphometric analysis of fish traits.
Stelios has a strong interest in molecular biology techniques and their application in wild and farmed marine living organisms. His involvement in the different activities and research programs held by IMBG, HCMR allowed him to obtain extensive knowledge on different methods and techniques used in molecular evolution laboratories.
His main responsibility is to carry out specific scientific procedures that will allow researchers to perform further analyses in the laboratory.
Typical tasks include: performing laboratory tests in order to produce reliable and precise data to support scientific investigations, sample preparation for laboratory analysis, including sample preservation and cataloging, preparation, optimization, and analyses of PCR reactions.
Building on his electronic engineering background he is also constructing, maintaining and operating standard laboratory equipment, for example centrifuges, titrators, pipetting machines and pH meters ensuring the laboratory is well-stocked.
Stelios is always keeping up to date with technical developments, especially those which can save time and improve reliability, following and ensuring strict safety procedures and safety checks.
He is also involved in the ordering of lab equipment and consumables to ensure continuous operations, and following up with suppliers and procurement where needed.
Moreover Stelios has experience in sampling from fish farms and field work for the needs of research programs.
Finally, besides his daily tasks, Stelios is in-charge of supporting visiting-researchers as well as visiting oversee students starting and carrying out their experiments when required.
Participation in Research Projects:
AQUAFIRST: FP6 â€“ EU project – SSP8-CT-2004-513692. Combined genetic and functional genomic approaches for stress and disease resistance marker assisted selection in fish and shellfish.
BRIDGEMAP (QLRT-2000-01797): Bridging genomes: an integrated genomic approach toward genetic improvement of aquacultured fish species.
BASSMAP (QLRT-2000-01701): Tools for the genetic improvement of sea bass. Construction and preliminary application of a medium density linkage and synteny map.
PENED ED 600 Introduction in genomic analysis methods in aquaculture: development of molecular tools and creation of Sparus aurata genetic map
EU DG XIV study 98/041. State of the stocks of European wreckfish (Polyprion americanus)
SWORDGEN: FP5 â€“ EU project – FAIR-CT98-3941. Fine genetic structure of swordfish (Xiphias gladius) in the Mediterranean and the Atlantic: Study by means of individual genetic tagging, using microsatellite DNA markers (coordinator) (FAIR CT 973941)
FAIR CT 96 1520. Cephalopod resources dynamics: patterns in environmental and genetic variation
FAIR CT 95 0421. Genetical bases and variability of physiological traits involved in growth in Crassostrea gigas
SELFDOTT: From capture based to SELF-sustained aquaculture and Domestication Of bluefin tuna, Thunnus thynnus
AQUAGENOME: Genomics in fish and shellfish: integration from research to aquaculture
Marine Genomics Europe (Network of Excellence) (CT-2003-505403): Implementation of high-throughput genomic approaches to investigate the functioning of marine ecosystems and the biology of marine organisms
PENEK (Cyprus): Management and genetic analysis of gilthead sea bream stocks
HOMSIR (QLK5-CT-1999-01438) A multidisciplinary approach using genetic markers and biological tags in horse mackerel (Trachurus trachurus)stock structure analysis.
SARDYN (Q5RS-2002-00818) Sardine Dynamics and Stock Structure in the North-east Atlantic
Panagiotis Kasapidis, Serge Planes, Veronique Laurent, Ricardo Quinta, Ana Teia, Philippe Lenfant, Stelios Darivianakis, Vasso Terzoglou, Giorgos Kotoulas and Antonios Magoulas